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验证数据展示

  • WB analysis of C6 using 60273-1-Ig

    WB analysis of C6 using 60273-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 60273-1-Ig

    IF Staining of hTERT-RPE1 using 60273-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CRYBB1 antibody (60273-1-Ig, Clone: 6F12B12 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CRYBB1 Monoclonal antibody

CRYBB1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 60273-1-Ig

产品说明书

CloneNo. 6F12B12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Beta B1 crystallin, Beta crystallin B1, CATCN3, CRYBB1, crystallin, beta B1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC6 cells, mouse eye tissue
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60273-1-Ig targets CRYBB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CRYBB1 fusion protein Ag4722 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 crystallin, beta B1
别名 Beta B1 crystallin, Beta crystallin B1, CATCN3, CRYBB1, crystallin, beta B1
计算分子量 252 aa, 28 kDa
观测分子量 30 kDa
GenBank蛋白编号BC036790
基因名称 CRYBB1
Gene ID (NCBI) 1414
RRIDAB_2881393
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP53674
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mutation in CRYBB1 is associated with congenital Cataract-Microcornea Syndrome.

实验方案

Product Specific Protocols
WB protocol for CRYBB1 antibody 60273-1-IgDownload protocol
IF protocol for CRYBB1 antibody 60273-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols