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CRYGS Polyclonal antibody

CRYGS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 16542-1-AP

Print datasheet

Synonyms

Beta crystallin S, CRYG8, CRYGS, crystallin, gamma S, Gamma crystallin S, Gamma S crystallin, GRYG8

验证数据展示

  • WB analysis using 16542-1-AP

    WB analysis using 16542-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16542-1-AP (CRYGS antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat eye tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16542-1-AP targets CRYGS in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen CRYGS fusion protein Ag9868 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name crystallin, gamma S
Synonyms Beta crystallin S, CRYG8, CRYGS, crystallin, gamma S, Gamma crystallin S, Gamma S crystallin, GRYG8
Calculated Molecular Weight 178 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC070241
Gene Symbol CRYGS
Gene ID (NCBI) 1427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRYGS (Gamma-S crystallin), also called CRYG8, belongs to the gamma-crystallins (PMID: 7733876; 23199295). Three major classes of crystallins, alpha, beta, and gamma, are common to the eye lens throughout all vertebrates. The beta and gamma classes are made up of homologous proteins and constitute the beta/gamma crystallin superfamily. CRYGS is expressed late but abundantly in the ocular lens (PMID: 1445197). It is also expressed outside the lens, particularly in the mature retina and cornea. In contrast to the beta-crystallins which associate in various combinations to form low or high molecular weight aggregates, CRYGS is, like the other gamma-crystallins, a monomeric protein (PMID: 16141006).

实验方案

Product Specific Protocols
WB protocol for CRYGS antibody 16542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols