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CRYZL1 Polyclonal antibody

CRYZL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16894-1-AP

Print datasheet

Synonyms

4P11, CRYZL1, Protein 4P11, QOH 1, Zeta crystallin homolog

验证数据展示

  • WB analysis of COS-7 using 16894-1-AP

    WB analysis of COS-7 using 16894-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 16894-1-AP (CRYZL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 16894-1-AP

    IHC staining of human ovary tumor using 16894-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 16894-1-AP

    IHC staining of human ovary tumor using 16894-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOS-7 cells, MCF-7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16894-1-AP targets CRYZL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat, monkey
Immunogen CRYZL1 fusion protein Ag10419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name crystallin, zeta (quinone reductase)-like 1
Synonyms 4P11, CRYZL1, Protein 4P11, QOH 1, Zeta crystallin homolog
Calculated Molecular Weight 349 aa, 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC013155
Gene Symbol CRYZL1
Gene ID (NCBI) 9946
RRIDAB_2878329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95825
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRYZL1 antibody 16894-1-APDownload protocol
IHC protocol for CRYZL1 antibody 16894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols