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验证数据展示

  • WB analysis using 31704-1-AP

    WB analysis using 31704-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 31704-1-AP (CSDA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of KYSE-30 using 31704-1-AP

    WB analysis of KYSE-30 using 31704-1-AP

    WB result of CSDA antibody (31704-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CSDA knockout KYSE-30 cells.

  • IF Staining of U2OS using 31704-1-AP

    IF Staining of U2OS using 31704-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CSDA antibody (31704-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Featured Product
  • KD/KO验证

CSDA Polyclonal antibody

CSDA Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31704-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Y-box-binding protein 3, Single-strand DNA-binding protein NF-GMB, DNA-binding protein A, DBPA, Cold shock domain-containing protein A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, KYSE-30 cells, mouse liver tissue, mouse skeletal muscle tissue
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31704-1-AP targets CSDA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CSDA fusion protein Ag36519 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cold shock domain protein A
别名 Y-box-binding protein 3, Single-strand DNA-binding protein NF-GMB, DNA-binding protein A, DBPA, Cold shock domain-containing protein A
计算分子量 40 kDa
观测分子量60-68 kDa
GenBank蛋白编号BC008801
基因名称 CSDA
Gene ID (NCBI) 8531
RRIDAB_3670088
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP16989
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSDA (Cold shock domain protein A), also known as DBPA (DNA-binding protein A), YBX3 (Y-box protein 3), is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE). CSDA is ubiquitous expressed in human skeletal muscle, heart, and decidual cells, which is thought to a multifunctional epithelial-specific protein involved in gene transcription, DNA repair, and interaction with other proteins (PMID: 33816248). CSDA is a nucleic-acid-binding protein that directly regulates gene expression by using different mechanisms, and acts as a translational repressor (e.g., MYH9 and SPTBN1) and positive regulator of mRNA stability (PMID: 31189097). Western blot analysis detected CDSA at a range of 50~70 kDa due to the anomalous mobility of Y-box proteins (PMID: 38255791).

实验方案

Product Specific Protocols
WB protocol for CSDA antibody 31704-1-APDownload protocol
IF protocol for CSDA antibody 31704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols