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验证数据展示

  • WB analysis using 67306-1-Ig

    WB analysis using 67306-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67306-1-Ig (CSE1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67306-1-Ig

    IHC staining of human colon cancer using 67306-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67306-1-Ig (CSE1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CSE1L antibody included
  • IHC staining of human colon cancer using 67306-1-Ig

    IHC staining of human colon cancer using 67306-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67306-1-Ig (CSE1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CSE1L antibody included
  • IF Staining of human colon cancer using 67306-1-Ig

    IF Staining of human colon cancer using 67306-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CSE1L antibody (67306-1-Ig, Clone: 1G1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human colon cancer using 67306-1-Ig

    IF Staining of human colon cancer using 67306-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CSE1L antibody (67306-1-Ig, Clone: 1G1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CSE1L Monoclonal antibody

CSE1L Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67306-1-Ig

产品说明书

CloneNo. 1G1A11

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells, Jurkat cells, HepG2 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CSE1L Ready-To-Use IHC Kit. CSE1L primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67306-1-Ig targets CSE1L in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 CSE1L fusion protein Ag29131 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CSE1 chromosome segregation 1-like (yeast)
别名 CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2
计算分子量 971 aa, 110 kDa
观测分子量 100 kDa
GenBank蛋白编号BC109313
基因名称 CSE1L
Gene ID (NCBI) 1434
RRIDAB_2882568
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55060
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CSE1L antibody 67306-1-IgDownload protocol
IHC protocol for CSE1L antibody 67306-1-IgDownload protocol
IF protocol for CSE1L antibody 67306-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols