验证数据展示
经过测试的应用
| Positive IHC detected in | mouse brain tissue, human brain tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 6 publications below |
| IF | See 1 publications below |
产品信息
25949-1-AP targets CSF1R/CD115 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | CSF1R/CD115 fusion protein Ag22845 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | colony stimulating factor 1 receptor |
| 别名 | CSF1R, CSF-1R, CSF-1 receptor, CSF 1 R, CD115 |
| 计算分子量 | 972 aa, 108 kDa |
| GenBank蛋白编号 | BC047521 |
| 基因名称 | CSF1R/CD115 |
| Gene ID (NCBI) | 1436 |
| RRID | AB_2880306 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07333 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CSF1R/CD115 antibody 25949-1-AP | Download protocol |
| IF protocol for CSF1R/CD115 antibody 25949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arthritis Res Ther Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies. | ||
CNS Neurosci Ther A novel chemokine-based signature for prediction of prognosis and therapeutic response in glioma | ||
Front Genet Human placenta-based genome-wide mRNA sequencing to identify TEK/IGF1/CSF1/ANGPT2 as crucial segments in the pathogenesis of pre-eclampsia | ||
J Immunol Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues | ||
Int J Exp Pathol Inhibition of inflammation and infiltration of M2 macrophages in NSCLC through the ATF3/CSF1 axis: Role of miR-27a-3p |
