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验证数据展示

  • WB analysis using 84477-6-RR

    WB analysis using 84477-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84477-6-RR (CSF1R antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 84477-6-RR

    IF Staining of THP-1 using 84477-6-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF1R antibody (84477-6-RR, Clone: 241675D4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84477-6-RR

    Affinity and Kinetic Characterization of 84477-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84477-6-RR against Human CSF1R were performed. The affinity constant is 0.775 nM.

CSF1R/CD115 Recombinant antibody

CSF1R/CD115 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84477-6-RR

产品说明书

CloneNo. 241675D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CSF1R, CSF-1 receptor, CSF 1 R, CD115, C FMS

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84477-6-RR targets CSF1R/CD115 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 1 receptor
别名 CSF1R, CSF-1 receptor, CSF 1 R, CD115, C FMS
计算分子量 972 aa, 108 kDa
观测分子量160 kDa
GenBank蛋白编号BC047521
基因名称 CSF1R/CD115
Gene ID (NCBI) 1436
RRIDAB_3671998
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP07333
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

实验方案

Product Specific Protocols
WB protocol for CSF1R/CD115 antibody 84477-6-RRDownload protocol
IF protocol for CSF1R/CD115 antibody 84477-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols