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验证数据展示

  • WB analysis of PC-3 using 26913-1-AP

    WB analysis of PC-3 using 26913-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26913-1-AP (CSGALNACT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 26913-1-AP

    IP experiment of PC-3 using 26913-1-AP

    IP result of anti-CSGALNACT1 (IP:26913-1-AP, 4ug; Detection:26913-1-AP 1:1000) with PC-3 cells lysate 3200ug.

CSGALNACT1 Polyclonal antibody

CSGALNACT1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 26913-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, ELISA

别名

beta4GalNAcT, Beta4GalNAcT 1, ChGn, CSGalNAcT 1, CSGALNACT1, GALNACT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, MCF-7 cells
Positive IP detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

26913-1-AP targets CSGALNACT1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原 CSGALNACT1 fusion protein Ag25455 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chondroitin sulfate N-acetylgalactosaminyltransferase 1
别名 beta4GalNAcT, Beta4GalNAcT 1, ChGn, CSGalNAcT 1, CSGALNACT1, GALNACT1
计算分子量 61 kDa
观测分子量 61-68 kDa
GenBank蛋白编号BC060772
基因名称 CSGALNACT1
Gene ID (NCBI) 55790
RRIDAB_2880682
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDX6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSGalNAcT-1 (chondroitin sulfate N-acetylgalactosaminyltransferase-1), catalyzing the transfer of a GalNAc residue onto the nonreducing end of GlcA, is a key glycosyltransferase for chondroitin sulfate (CS) biosynthesis in cartilage. Abnormal expression of CSGalNAcT-1 in cartilage has been linked to diseases like osteoarthritis.

实验方案

Product Specific Protocols
WB protocol for CSGALNACT1 antibody 26913-1-APDownload protocol
IP protocol for CSGALNACT1 antibody 26913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Br J Pharmacol

Chondroitin sulfate proteoglycan is a potential target of memantine to improve cognitive function via the promotion of adult neurogenesis.

Authors - Shoichiro Maeda
View Article
WB

Methods Mol Biol

Bacterial Amyloids.

Authors - Margery L Evans
View Article