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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis of Jurkat using 66115-1-Ig (same clone as 66115-1-PBS)

    WB analysis of Jurkat using 66115-1-Ig (same clone as 66115-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66115-1-Ig (same clone as 66115-1-PBS)

    IF Staining of A431 using 66115-1-Ig (same clone as 66115-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CSK antibody (66115-1-Ig, Clone: 3B12C5 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

CSK Monoclonal antibody, PBS Only

CSK Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66115-1-PBS

产品说明书

CloneNo. 3B12C5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Protein-tyrosine kinase CYL, EC:2.7.10.2, C-Src kinase, Csk A, C Src kinase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CSK Ready-To-Use IHC Kit. CSK primary antibody included.

产品信息

66115-1-PBS targets CSK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CSK fusion protein Ag12103 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 c-src tyrosine kinase
别名 Protein-tyrosine kinase CYL, EC:2.7.10.2, C-Src kinase, Csk A, C Src kinase
计算分子量 450 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC106073
基因名称 CSK
Gene ID (NCBI) 1445
RRIDAB_2881514
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41240
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).