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CSK Monoclonal antibody, PBS Only

CSK Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat No : 66115-1-PBS

Print datasheet

Synonyms

C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK

验证数据展示

  • WB analysis of Jurkat using 66115-1-Ig (same clone as 66115-1-PBS)

    WB analysis of Jurkat using 66115-1-Ig (same clone as 66115-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66115-1-Ig (same clone as 66115-1-PBS)

    IF Staining of A431 using 66115-1-Ig (same clone as 66115-1-PBS)

    Immunofluorescent analysis of A431 cells using 66115-1-Ig (CSK antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

产品信息

66115-1-PBS targets CSK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CSK fusion protein Ag12103 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name c-src tyrosine kinase
Synonyms C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC106073
Gene Symbol CSK
Gene ID (NCBI) 1445
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41240
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).