CoraLite®594-conjugated CSK Monoclonal antibody

CSK Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3B12C5

Cat no : CL594-66115

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Synonyms

Protein-tyrosine kinase CYL, EC:2.7.10.2, C-Src kinase, Csk A, C Src kinase



经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CSK Ready-To-Use IHC Kit. CSK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66115-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66115 targets CSK in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen CSK fusion protein Ag12103 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name c-src tyrosine kinase
Synonyms Protein-tyrosine kinase CYL, EC:2.7.10.2, C-Src kinase, Csk A, C Src kinase
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC106073
Gene Symbol CSK
Gene ID (NCBI) 1445
RRIDAB_2883516
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41240
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).

实验方案

Product Specific Protocols
IF protocol for CL594 CSK antibody CL594-66115Download protocol
Standard Protocols
Click here to view our Standard Protocols