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验证数据展示

  • IF Staining of human ovary tumor using 66435-1-Ig (same clone as 66435-1-PBS)

    IF Staining of human ovary tumor using 66435-1-Ig (same clone as 66435-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CSN2 antibody (66435-1-Ig, Clone: 2H10E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66435-1-Ig (same clone as 66435-1-PBS)

    WB analysis of NIH/3T3 using 66435-1-Ig (same clone as 66435-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66435-1-Ig (same clone as 66435-1-PBS)

    IHC staining of human ovary tumor using 66435-1-Ig (same clone as 66435-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (CSN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COPS2 antibody included
  • IHC staining of human ovary tumor using 66435-1-Ig (same clone as 66435-1-PBS)

    IHC staining of human ovary tumor using 66435-1-Ig (same clone as 66435-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (COPS2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COPS2 antibody included

CSN2 Monoclonal antibody, PBS Only

CSN2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66435-1-PBS

产品说明书

CloneNo. 2H10E6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

ALIEN, Alien homolog, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy COPS2 Ready-To-Use IHC Kit. COPS2 primary antibody included.

产品信息

66435-1-PBS targets CSN2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CSN2 fusion protein Ag1415 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
别名 ALIEN, Alien homolog, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC012629
基因名称 COPS2
Gene ID (NCBI) 9318
RRIDAB_2881805
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61201
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.