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CSNK1A1 Monoclonal antibody

CSNK1A1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E2E7

Cat No : 68434-1-Ig

Print datasheet

Synonyms

casein kinase 1, alpha 1, Casein kinase I isoform alpha, CK1, CKI alpha, CSNK1A1, HLCDGP1, PRO2975

验证数据展示

  • WB analysis using 68434-1-Ig

    WB analysis using 68434-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68434-1-Ig (CSNK1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68434-1-Ig targets CSNK1A1 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name casein kinase 1, alpha 1
Synonyms casein kinase 1, alpha 1, Casein kinase I isoform alpha, CK1, CKI alpha, CSNK1A1, HLCDGP1, PRO2975
Calculated Molecular Weight 42 kDa
Observed Molecular Weight30-40 kDa
GenBank Accession NumberNM_001892
Gene Symbol CSNK1A1
Gene ID (NCBI) 1452
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.

实验方案

Product Specific Protocols
WB protocol for CSNK1A1 antibody 68434-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols