• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CSNK1A1 Monoclonal antibody

CSNK1A1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E2E7

Cat no : 68434-1-Ig

Print datasheet

Synonyms

CSNK, CKI-alpha, CKI alpha, CK1α, CK1

验证数据展示

  • WB analysis using 68434-1-Ig

    WB analysis using 68434-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68434-1-Ig (CSNK1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CSNK1A1 Ready-To-Use IHC Kit. CSNK1A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68434-1-Ig targets CSNK1A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name casein kinase 1, alpha 1
Synonyms CSNK, CKI-alpha, CKI alpha, CK1α, CK1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight30-40 kDa
GenBank Accession NumberNM_001892
Gene Symbol CSNK1A1
Gene ID (NCBI) 1452
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.

实验方案

Product Specific Protocols
WB protocol for CSNK1A1 antibody 68434-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols