Casein Kinase 1 Epsilon Polyclonal antibody
Casein Kinase 1 Epsilon Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CSNK1E Ready-To-Use IHC Kit. CSNK1E primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11230-1-AP targets Casein Kinase 1 Epsilon in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Casein Kinase 1 Epsilon fusion protein Ag1742 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | casein kinase 1, epsilon |
| Synonyms | Casein Kinase 1 epsilon, casein kinase 1, epsilon, CKI epsilon, CKIe, CSNK1E, HCKIE |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 46-52 kDa |
| GenBank Accession Number | BC006490 |
| Gene Symbol | CK1 epsilon |
| Gene ID (NCBI) | 1454 |
| RRID | AB_2084501 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49674 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSNK1E(Casein kinase I isoform epsilon) is also named as CKIe, CKI-epsilon and belongs to the casein kinase I subfamily. It plays a significant role in delaying the negative feedback signal within the transcription-translation-based autoregulatory loop that composes the core of the circadian mechanism and makes an ideal target for pharmaceutical compounds influencing circadian rhythms, sleep, and jet lag, as well as other physiologic and metabolic processes under circadian regulation(PMID:10775102). It can be autophosphorylated(PMID:19690332).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Casein Kinase 1 Epsilon antibody 11230-1-AP | Download protocol |
| IHC protocol for Casein Kinase 1 Epsilon antibody 11230-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
