验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CSNK1E Ready-To-Use IHC Kit. CSNK1E primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11230-1-AP targets Casein Kinase 1 Epsilon in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | Casein Kinase 1 Epsilon fusion protein Ag1742 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | casein kinase 1, epsilon |
| 别名 | Casein Kinase 1 epsilon, casein kinase 1, epsilon, CKI epsilon, CKIe, CSNK1E, HCKIE |
| 计算分子量 | 47 kDa |
| 观测分子量 | 46-52 kDa |
| GenBank蛋白编号 | BC006490 |
| 基因名称 | CK1 epsilon |
| Gene ID (NCBI) | 1454 |
| RRID | AB_2084501 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49674 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSNK1E(Casein kinase I isoform epsilon) is also named as CKIe, CKI-epsilon and belongs to the casein kinase I subfamily. It plays a significant role in delaying the negative feedback signal within the transcription-translation-based autoregulatory loop that composes the core of the circadian mechanism and makes an ideal target for pharmaceutical compounds influencing circadian rhythms, sleep, and jet lag, as well as other physiologic and metabolic processes under circadian regulation(PMID:10775102). It can be autophosphorylated(PMID:19690332).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Casein Kinase 1 Epsilon antibody 11230-1-AP | Download protocol |
| IHC protocol for Casein Kinase 1 Epsilon antibody 11230-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
