验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, COS-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IP | See 1 publications below |
产品信息
16384-1-AP targets CSNK1G1 in WB, IP, IHC, ELISA applications and shows reactivity with human, monkey samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, monkey |
| 文献引用反应性 | human |
| 免疫原 | CSNK1G1 fusion protein Ag9395 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | casein kinase 1, gamma 1 |
| 别名 | casein kinase 1, gamma 1, CKI gamma 1, CSNK1G1 |
| 计算分子量 | 422 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC017236 |
| 基因名称 | CSNK1G1 |
| Gene ID (NCBI) | 53944 |
| RRID | AB_2087300 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HCP0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSNK1G1 antibody 16384-1-AP | Download protocol |
| IHC protocol for CSNK1G1 antibody 16384-1-AP | Download protocol |
| IP protocol for CSNK1G1 antibody 16384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
