验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, PC-3 cells, K-562 cells, NIH/3T3 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10992-1-AP targets CSNK2A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CSNK2A1 fusion protein Ag1452 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | casein kinase 2, alpha 1 polypeptide |
| 别名 | CSNK2A 1, CKII, CK2α, CK2A1, CK2a |
| 计算分子量 | 45 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC011668 |
| 基因名称 | CSNK2A1 |
| Gene ID (NCBI) | 1457 |
| RRID | AB_2245531 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P68400 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| IHC protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| IF protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| IP protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy | ||
Nucleic Acids Res CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling
| ||
Nucleic Acids Res CK2 kinase-mediated PHF8 phosphorylation controls TopBP1 stability to regulate DNA replication.
| ||
Nucleic Acids Res ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters. | ||
Redox Biol Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury. | ||
Cancer Lett CSNK2A1 confers gemcitabine resistance to pancreatic ductal adenocarcinoma via inducing autophagy |
