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  • Featured Product
  • KD/KO Validated

CSNK2B Monoclonal antibody, PBS Only

CSNK2B Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1B5A6

Cat No : 67866-1-PBS

Print datasheet

Synonyms

Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

验证数据展示

  • WB analysis using 67866-1-Ig (same clone as 67866-1-PBS)

    WB analysis using 67866-1-Ig (same clone as 67866-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67866-1-Ig (CSNK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 67866-1-Ig (same clone as 67866-1-PBS)

    WB analysis of MCF-7 using 67866-1-Ig (same clone as 67866-1-PBS)

    WB result of CSNK2B antibody (67866-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK2B transfected MCF-7 cells. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67866-1-Ig (same clone as 67866-1-PBS)

    IF Staining of HeLa using 67866-1-Ig (same clone as 67866-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSNK2B antibody (67866-1-Ig, Clone: 1B5A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

    IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSNK2B antibody included
  • IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

    IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSNK2B antibody included
Planning an IHC experiment? We recommend our IHCeasy CSNK2B Ready-To-Use IHC Kit. CSNK2B primary antibody included.

产品信息

67866-1-PBS targets CSNK2B in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen CSNK2B fusion protein Ag19180 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name casein kinase 2, beta polypeptide
Synonyms Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a
Calculated Molecular Weight 215 aa, 25 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC112017
Gene Symbol CSNK2B
Gene ID (NCBI) 1460
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP67870
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.