验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, A431 cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CSNK2B Ready-To-Use IHC Kit. CSNK2B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20234-1-AP targets CSNK2B in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | casein kinase 2, beta polypeptide |
| 别名 | CSNK2B-specific, Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | NM_001320 |
| 基因名称 | CSNK2B |
| Gene ID (NCBI) | 1460 |
| RRID | AB_10733246 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P67870 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSNK2B, also named as CK2N, G5A nd CK II beta, belongs to the casein kinase 2 subunit beta family. CSNK2B participates in Wnt signaling. It plays a complex role in regulating the basal catalytic activity of the alpha subunit. The antibody is specific to CSNK2B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSNK2B antibody 20234-1-AP | Download protocol |
| IHC protocol for CSNK2B antibody 20234-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury. | ||
Phytomedicine Spiropachysine A suppresses hepatocellular carcinoma proliferation by inducing methuosis in vitro and in vivo. | ||
Front Mol Neurosci Lovastatin Alleviates α-Synuclein Aggregation and Phosphorylation in Cellular Models of Synucleinopathy. | ||
J Cell Commun Signal CSNK2B contributes to colorectal cancer cell proliferation by activating the mTOR signaling.
| ||
Exp Cell Res Oocyte-specific deletion of Gsα induces oxidative stress and deteriorates oocyte quality in mice.
| ||
J Exp Clin Cancer Res Overexpression of NELFE contributes to gastric cancer progression via Wnt/β-catenin signaling-mediated activation of CSNK2B expression.
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