CSPG4 Polyclonal antibody

CSPG4 Polyclonal Antibody for WB, ELISA
Cat No. 55027-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA and More (1)

NG2, MSK16, Melanoma-associated chondroitin sulfate proteoglycan, MCSPG, MCSP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55027-1-AP targets CSPG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chondroitin sulfate proteoglycan 4
别名 NG2, MSK16, Melanoma-associated chondroitin sulfate proteoglycan, MCSPG, MCSP
计算分子量 251 kDa
观测分子量 240-250 kDa, 450kDa
GenBank蛋白编号NM_001897
基因名称 CSPG4
Gene ID (NCBI) 1464
RRIDAB_10791888
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UVK1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSPG4, also named as HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16 and NG2, is a proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. CSPG4 may inhibit neurite outgrowth and growth cone collapse during axon regeneration. It is cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. CSPG4 may regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. It modulates the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. CSPG4 functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. It promotes retraction fiber formation and cell polarization through Rho GTPase activation and stimulates alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. CSPG4 activates FAK and ERK1/ERK2 signaling cascades. The antibody is specific to CSPG4.

实验方案

Product Specific Protocols
WB protocol for CSPG4 antibody 55027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.

Authors - Steinberg Florian F
mouse

Nat Commun

FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment

Authors - Zongchao Sun
mouseWB

J Pineal Res

Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.

Authors - Lirong Liang
mouseWB

Glia

ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.

Authors - Lukasz Mateusz Szewczyk
mouse

Commun Biol

Complete remission of diabetes with a transient HDAC inhibitor and insulin in streptozotocin mice

Authors - Hideto Kojima
mouse

ACS Biomater Sci Eng

Acid Neutralization by Composite Lysine Nanoparticles for Spinal Cord Injury Recovery through Mitigating Mitochondrial Dysfunction

Authors - Qiuchen Wang
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