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Conjugate
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CSPG4 Polyclonal antibody

CSPG4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31623-1-AP

Print datasheet

Synonyms

NG2, MSK16, Melanoma-associated chondroitin sulfate proteoglycan, MCSPG, MCSP

验证数据展示

  • WB analysis using 31623-1-AP

    WB analysis using 31623-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31623-1-AP (CSPG4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31623-1-AP targets CSPG4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chondroitin sulfate proteoglycan 4
Synonyms NG2, MSK16, Melanoma-associated chondroitin sulfate proteoglycan, MCSPG, MCSP
Calculated Molecular Weight251kd
Observed Molecular Weight 250-300 kDa
GenBank Accession NumberNM_001897.5
Gene Symbol CSPG4
Gene ID (NCBI) 1464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6UVK1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSPG4 is a proteoglycan that plays a role in cell proliferation and migration, stimulates endothelial cell motility during microvascular morphogenesis and may also inhibit neurite outgrowth and growth cone collapse during axon regeneration. CSPG4 is expressed on more than 90% of human melanoma tissues and cultured cells. It is a target of specific diagnosis and immunotherapy with antiidiotypic antibodies due, in part, to its roles in growth control, adhesion, cell-substratum interactions, and cell-cell contacts (PMID: 10587647; 11278606).

实验方案

Product Specific Protocols
WB protocol for CSPG4 antibody 31623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols