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验证数据展示

  • WB analysis using 15537-1-AP

    WB analysis using 15537-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15537-1-AP (CSTF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15537-1-AP

    IF Staining of HeLa using 15537-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15537-1-AP (CSTF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 15537-1-AP

    IP experiment of HeLa using 15537-1-AP

    IP result of anti-CSTF1 (IP:15537-1-AP, 4ug; Detection:15537-1-AP 1:500) with HeLa cells lysate 680 ug.

CSTF1 Polyclonal antibody

CSTF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 15537-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

CstF-50, CstF50, CF-1 50 kDa subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, A549 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15537-1-AP targets CSTF1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 CSTF1 fusion protein Ag7874 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
别名 CstF-50, CstF50, CF-1 50 kDa subunit
计算分子量 48 kDa
观测分子量 50 kDa
GenBank蛋白编号BC007425
基因名称 CSTF1
Gene ID (NCBI) 1477
RRIDAB_2261143
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05048
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 unit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD).

实验方案

Product Specific Protocols
WB protocol for CSTF1 antibody 15537-1-APDownload protocol
IF protocol for CSTF1 antibody 15537-1-APDownload protocol
IP protocol for CSTF1 antibody 15537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols