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CSTF1 Polyclonal antibody

CSTF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15537-1-AP

Print datasheet

Synonyms

CstF-50, CstF50, CF-1 50 kDa subunit

验证数据展示

  • WB analysis using 15537-1-AP

    WB analysis using 15537-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15537-1-AP (CSTF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15537-1-AP

    IF Staining of HeLa using 15537-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15537-1-AP (CSTF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 15537-1-AP

    IP experiment of HeLa using 15537-1-AP

    IP result of anti-CSTF1 (IP:15537-1-AP, 4ug; Detection:15537-1-AP 1:500) with HeLa cells lysate 680 ug.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, A549 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15537-1-AP targets CSTF1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen CSTF1 fusion protein Ag7874 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Synonyms CstF-50, CstF50, CF-1 50 kDa subunit
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC007425
Gene Symbol CSTF1
Gene ID (NCBI) 1477
RRIDAB_2261143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05048
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 unit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD).

实验方案

Product Specific Protocols
WB protocol for CSTF1 antibody 15537-1-APDownload protocol
IF protocol for CSTF1 antibody 15537-1-APDownload protocol
IP protocol for CSTF1 antibody 15537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols