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CSTF2T Polyclonal antibody

CSTF2T Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14206-1-AP

Print datasheet

Synonyms

TauCstF-64, TauCstF 64, KIAA0689, CstF 64T, Cleavage stimulation factor 64 kDa subunit tau variant

验证数据展示

  • WB analysis using 14206-1-AP

    WB analysis using 14206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14206-1-AP (CSTF2T antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 14206-1-AP

    IHC staining of human prostate cancer using 14206-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14206-1-AP (CSTF2T antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human prostate cancer using 14206-1-AP

    IHC staining of human prostate cancer using 14206-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14206-1-AP (CSTF2T antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of mouse brain using 14206-1-AP

    IP experiment of mouse brain using 14206-1-AP

    IP result of anti-CSTF2T (IP:14206-1-AP, 4ug; Detection:14206-1-AP 1:1000) with mouse brain tissue lysate 5200ug.

  • IF Staining of HepG2 using 14206-1-AP

    IF Staining of HepG2 using 14206-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14206-1-AP (CSTF2T antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, rat brain tissue, human brain tissue, HeLa cells, HepG2 cells, mouse liver tissue
Positive IP detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14206-1-AP targets CSTF2T in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CSTF2T fusion protein Ag5411 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant
Synonyms TauCstF-64, TauCstF 64, KIAA0689, CstF 64T, Cleavage stimulation factor 64 kDa subunit tau variant
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC028239
Gene Symbol CSTF2T
Gene ID (NCBI) 23283
RRIDAB_2086478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0L4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CSTF2T antibody 14206-1-APDownload protocol
IHC protocol for CSTF2T antibody 14206-1-APDownload protocol
IF protocol for CSTF2T antibody 14206-1-APDownload protocol
IP protocol for CSTF2T antibody 14206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols