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  • Featured Product
  • KD/KO Validated

CSTF3 Polyclonal antibody

CSTF3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24290-1-AP

Print datasheet

Synonyms

CF 1 77 kDa subunit, CSTF 77, CSTF 77 kDa subunit, CSTF3

验证数据展示

  • WB analysis using 24290-1-AP

    WB analysis using 24290-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24290-1-AP (CSTF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 24290-1-AP

    IP experiment of A431 using 24290-1-AP

    IP result of anti-CSTF3 (IP:24290-1-AP, 4ug; Detection:24290-1-AP 1:500) with A431 cells lysate 1200ug.

经过测试的应用

Positive WB detected inA431 cells, K-562 cells, HEK-293 cells
Positive IP detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CSTF3 Ready-To-Use IHC Kit. CSTF3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24290-1-AP targets CSTF3 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CSTF3 fusion protein Ag17930 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
Synonyms CF 1 77 kDa subunit, CSTF 77, CSTF 77 kDa subunit, CSTF3
Calculated Molecular Weight 717 aa, 83 kDa
Observed Molecular Weight 77-83 kDa
GenBank Accession NumberBC059948
Gene Symbol CSTF3
Gene ID (NCBI) 1479
RRIDAB_2879475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12996
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CSTF3 antibody 24290-1-APDownload protocol
IP protocol for CSTF3 antibody 24290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF,IP

iScience

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Authors - Ziqian Min
  • KD Validated
View Article
humanWB

Comput Struct Biotechnol J

Alternative polyadenylation regulates the translation of metabolic and inflammation-related proteins in adipose tissue of gestational diabetes mellitus

Authors - Bingnan Chen
View Article
humanIF

Cell Death Dis

CSTF3 contributes to platinum resistance in ovarian cancer through alternative polyadenylation of lncRNA NEAT1 and generating the short isoform NEAT1_1

Authors - Xin Luo
View Article