• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FLJ23584 Polyclonal antibody

FLJ23584 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21143-1-AP

Print datasheet

Synonyms

C22orf46, CTA 216E10.6, CTA-216E10.6, FLJ23584, hypothetical FLJ23584

验证数据展示

  • WB analysis of HepG2 using 21143-1-AP

    WB analysis of HepG2 using 21143-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (FLJ23584 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 21143-1-AP

    IHC staining of human brain using 21143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 21143-1-AP

    IHC staining of human brain using 21143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 21143-1-AP

    IF Staining of HepG2 using 21143-1-AP

    Immunofluorescent analysis of HepG2 cells using 21143-1-AP (FLJ23584 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, L02 cells, mouse liver tissue
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21143-1-AP is FLJ23584 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FLJ23584 fusion protein Ag15647 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hypothetical FLJ23584
Synonyms C22orf46, CTA 216E10.6, CTA-216E10.6, FLJ23584, hypothetical FLJ23584
Calculated Molecular Weight 234 aa, 26 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC007210
Gene Symbol FLJ23584
Gene ID (NCBI) 79640
RRIDAB_10732689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDC9J442
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FLJ23584 is coded by the mRNA of chromosome 22 open reading frame 46. The reading frame is different to C22orf46. This antibody detects the hypothetical FLJ23584 protein.

实验方案

Product Specific Protocols
WB protocol for FLJ23584 antibody 21143-1-APDownload protocol
IHC protocol for FLJ23584 antibody 21143-1-APDownload protocol
IF protocol for FLJ23584 antibody 21143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols