验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, HT-1080 cells, NCI-H1299 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
19521-1-AP targets NY-ESO-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cancer/testis antigen 1A |
| 别名 | CTAG1A, Autoimmunogenic cancer/testis antigen NY-ESO-1, CTAG, CTAG1, ESO1 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 16-25 kDa |
| GenBank蛋白编号 | NM_139250 |
| 基因名称 | NY-ESO-1 |
| Gene ID (NCBI) | 246100 |
| RRID | AB_10638144 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78358 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTAG1A, also named as NY-ESO-1, CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B and CT6.1, belongs to the CTAG family.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NY-ESO-1 antibody 19521-1-AP | Download protocol |
| IHC protocol for NY-ESO-1 antibody 19521-1-AP | Download protocol |
| IF protocol for NY-ESO-1 antibody 19521-1-AP | Download protocol |
| IP protocol for NY-ESO-1 antibody 19521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis | ||
Cancer Immunol Res CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance | ||
Nat Cancer The IRENA lncRNA converts chemotherapy-polarized tumor-suppressing macrophages to tumor-promoting phenotypes in breast cancer. | ||
Cell Death Dis STING agonist diABZI enhances the cytotoxicity of T cell towards cancer cells |
