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NY-ESO-1 Polyclonal antibody

NY-ESO-1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19521-1-AP

Print datasheet

Synonyms

CTAG1A, LAGE2, ESO1, CTAG1, CTAG

验证数据展示

  • WB analysis of human testis using 19521-1-AP

    WB analysis of human testis using 19521-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19521-1-AP (CTAG1A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19521-1-AP

    IF Staining of HepG2 using 19521-1-AP

    Immunofluorescent analysis of HepG2 cells, using CTAG1A antibody 19521-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HepG2 using 19521-1-AP

    IP experiment of HepG2 using 19521-1-AP

    IP result of anti-NY-ESO-1 (IP:19521-1-AP, 4ug; Detection:19521-1-AP 1:500) with HepG2 cells lysate 3200ug.

经过测试的应用

Positive WB detected inhuman testis tissue, HT-1080 cells, NCI-H1299 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19521-1-AP targets NY-ESO-1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cancer/testis antigen 1A
Synonyms CTAG1A, LAGE2, ESO1, CTAG1, CTAG
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 16-25 kDa
GenBank Accession NumberNM_139250
Gene Symbol NY-ESO-1
Gene ID (NCBI) 246100
RRIDAB_10638144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78358
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTAG1A, also named as NY-ESO-1, CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B and CT6.1, belongs to the CTAG family.

实验方案

Product Specific Protocols
WB protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
IF protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
IP protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

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Authors - Liyan Lao
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humanWB

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humanWB

Signal Transduct Target Ther

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

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humanIF,WB

Cell Death Dis

STING agonist diABZI enhances the cytotoxicity of T cell towards cancer cells

Authors - Ling Wang
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