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MARK3 Polyclonal antibody

MARK3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12932-1-AP

Print datasheet

Synonyms

C TAK1, CTAK1, ELKL motif kinase 2, EMK 2, EMK2, KP78, MARK3, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR 1

验证数据展示

  • WB analysis of human brain using 12932-1-AP

    WB analysis of human brain using 12932-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12932-1-AP (MARK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12932-1-AP

    IP experiment of mouse brain using 12932-1-AP

    IP result of anti-MARK3 (IP:12932-1-AP, 3ug; Detection:12932-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman brain tissue, PC-3 cells
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12932-1-AP targets MARK3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MARK3 fusion protein Ag3805 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MAP/microtubule affinity-regulating kinase 3
Synonyms C TAK1, CTAK1, ELKL motif kinase 2, EMK 2, EMK2, KP78, MARK3, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR 1
Calculated Molecular Weight 78 kDa, 80 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC024773
Gene Symbol MARK3
Gene ID (NCBI) 4140
RRIDAB_10638613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27448
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MARK3 antibody 12932-1-APDownload protocol
IP protocol for MARK3 antibody 12932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols