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验证数据展示

  • WB analysis of human brain using 12932-1-AP

    WB analysis of human brain using 12932-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12932-1-AP (MARK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12932-1-AP

    IP experiment of mouse brain using 12932-1-AP

    IP result of anti-MARK3 (IP:12932-1-AP, 3ug; Detection:12932-1-AP 1:500) with mouse brain tissue lysate 4000ug.

MARK3 Polyclonal antibody

MARK3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12932-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

C TAK1, CTAK1, ELKL motif kinase 2, EMK 2, EMK2, KP78, MARK3, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, PC-3 cells
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12932-1-AP targets MARK3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MARK3 fusion protein Ag3805 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAP/microtubule affinity-regulating kinase 3
别名 C TAK1, CTAK1, ELKL motif kinase 2, EMK 2, EMK2, KP78, MARK3, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR 1
计算分子量 78 kDa, 80 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC024773
基因名称 MARK3
Gene ID (NCBI) 4140
RRIDAB_10638613
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27448
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MARK3 antibody 12932-1-APDownload protocol
IP protocol for MARK3 antibody 12932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols