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CTCF Polyclonal antibody

CTCF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30428-1-AP

Print datasheet

Synonyms

11 zinc finger protein, CCCTC binding factor, CTCF, CTCFL paralog, Transcriptional repressor CTCF

验证数据展示

  • WB analysis using 30428-1-AP

    WB analysis using 30428-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30428-1-AP (CTCF antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 30428-1-AP

    IP experiment of MCF-7 using 30428-1-AP

    IP result of anti-CTCF (IP:30428-1-AP, 4ug; Detection:30428-1-AP 1:8000) with MCF-7 cells lysate 1880 ug.

  • IHC staining of mouse lung using 30428-1-AP

    IHC staining of mouse lung using 30428-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30428-1-AP (CTCF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CTCF antibody included
  • IHC staining of mouse lung using 30428-1-AP

    IHC staining of mouse lung using 30428-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30428-1-AP (CTCF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CTCF antibody included
  • IF Staining of HepG2 using 30428-1-AP

    IF Staining of HepG2 using 30428-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTCF antibody (30428-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, RAW 264.7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CTCF Ready-To-Use IHC Kit. CTCF primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30428-1-AP targets CTCF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen CTCF fusion protein Ag32417 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CCCTC-binding factor (zinc finger protein)
Synonyms 11 zinc finger protein, CCCTC binding factor, CTCF, CTCFL paralog, Transcriptional repressor CTCF
Calculated Molecular Weight 83 kDa
Observed Molecular Weight110-150 kDa
GenBank Accession NumberBC014267
Gene Symbol CTCF
Gene ID (NCBI) 10664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49711
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcriptional insulators are DNA elements that set boundaries on the actions of enhancer and silencer elements and thereby organize the eukaryotic genome into regulatory domains. All vertebrate insulators appear to use the versatile CTCF protein. CTCF uses various combinations of its 11 zinc fingers to recognize a variety of unrelated DNA sequences. Once bound to DNA, CTCF can function as a transcriptional insulator, repressor, or activator, depending on the context of the binding site [PMID:12787766,15454938]. In vertebrates, this 11 zinc-finger protein is shown to be crucial in processes of epigenetic imprinting, X chromosome inactivation , and associated with various complex human diseases including cancer and diabetes [PMID:23139640]. The calcualted molecular weight of CTCF is 83 kDa, but stimulation of human corneal epithelial cells with hypoxic stress suppressed a high molecular mass form of CTCF (150 kDa), but not a lower molecular weight form of CTCF (130 kDa)(PMID: 22354964), and there are multiple isoforms of CTCF with molecular masses of 55, 70, 73, 80, 97, and 130 kDa have been observed (PMID: 12878173).

实验方案

Product Specific Protocols
WB protocol for CTCF antibody 30428-1-APDownload protocol
IHC protocol for CTCF antibody 30428-1-APDownload protocol
IF protocol for CTCF antibody 30428-1-APDownload protocol
IP protocol for CTCF antibody 30428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols