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CTDSP2 Polyclonal antibody

CTDSP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11116-1-AP

Print datasheet

Synonyms

CTDSP2, NIF2, NLI interacting factor 2, OS4, Protein OS 4, PSR2, SCP2, Small CTD phosphatase 2

验证数据展示

  • WB analysis of human kidney using 11116-1-AP

    WB analysis of human kidney using 11116-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11116-1-AP

    IHC staining of human pancreas cancer using 11116-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11116-1-AP (CTDSP2 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inhuman kidney tissue, HepG2 cells, K-562 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11116-1-AP targets CTDSP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CTDSP2 fusion protein Ag1601 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2
Synonyms CTDSP2, NIF2, NLI interacting factor 2, OS4, Protein OS 4, PSR2, SCP2, Small CTD phosphatase 2
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC065920
Gene Symbol CTDSP2
Gene ID (NCBI) 10106
RRIDAB_2087086
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14595
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTDSPs are C-class phosphatases that act on Ser 5 of the Pol II C-terminal domain (CTD) to decrease transcription [PMID:15681389]. CTDSP enzymatic activity is required for REST function, and knockdown of CTDSP induces the neuronal differentiation of mouse embryonic carcinoma P19 cells. CTDSP2 has been shown to enhance TGF-β signaling by the dephosphorylation of SMAD proteins and attenuates androgen receptor-mediated transcription [PMID:16724108]. It negatively regulates RNA polymerase II transcription by controlling the transition from initiation/capping to processive transcript elongation. It was recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells[PMID: 12721286].

实验方案

Product Specific Protocols
WB protocol for CTDSP2 antibody 11116-1-APDownload protocol
IHC protocol for CTDSP2 antibody 11116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols