验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, HeLa cells, HepG2 cells, human brain tissue, mouse eye tissue, mouse testis tissue, Y79 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human endometrial cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17532-1-AP targets CTDSPL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | CTDSPL2 fusion protein Ag11581 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2 |
| 别名 | HSPC129, HSPC058, EC:3.1.3.-, CTDSP-like 2, CTDSP like 2 |
| 计算分子量 | 466 aa, 53 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC035744 |
| 基因名称 | CTDSPL2 |
| Gene ID (NCBI) | 51496 |
| RRID | AB_2087204 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q05D32 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTD small phosphatase-like protein 2 (CTDSPL2) is also named as HSPC058 and HSPC129, belongs to the CTDSPL2 family. It is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase, whose specific CTD phosphatase activity was verified in vitro, and may regulate the dynamic phosphorylation of RNAP II CTD.( PMID: 17487459) It can obviously improve the expression of ε- and γ-globin genes in K562 cells and CD34+ cells derived from UCB.( PMID: 20932329) This antibody is a rabbit polyclonal antibody raised against a region of human CTDSPL2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CTDSPL2 antibody 17532-1-AP | Download protocol |
| IHC protocol for CTDSPL2 antibody 17532-1-AP | Download protocol |
| IF protocol for CTDSPL2 antibody 17532-1-AP | Download protocol |
| IP protocol for CTDSPL2 antibody 17532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Cell Biol CTD small phosphatase like 2 (CTDSPL2) can increase ε- and γ-globin gene expression in K562 cells and CD34+ cells derived from umbilical cord blood. | ||
Cell Death Discov CTDSPL2 promotes the progression of non-small lung cancer through PI3K/AKT signaling via JAK1
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