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CTHRC1 Polyclonal antibody

CTHRC1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 16534-1-AP

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Synonyms

UNQ762/PRO1550, Protein NMTC1, Collagen triple helix repeat-containing protein 1, collagen triple helix repeat containing 1



经过测试的应用

Positive WB detected inA375 cells, Jurkat cells, mouse lung tissue
Positive IP detected inA375 cells
Positive IF/ICC detected inA375 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16534-1-AP targets CTHRC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen CTHRC1 fusion protein Ag9812 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen triple helix repeat containing 1
Synonyms UNQ762/PRO1550, Protein NMTC1, Collagen triple helix repeat-containing protein 1, collagen triple helix repeat containing 1
Calculated Molecular Weight 243 aa, 26 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC014245
Gene Symbol CTHRC1
Gene ID (NCBI) 115908
RRIDAB_2087511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CG8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).

实验方案

Product Specific Protocols
WB protocol for CTHRC1 antibody 16534-1-APDownload protocol
IF protocol for CTHRC1 antibody 16534-1-APDownload protocol
IP protocol for CTHRC1 antibody 16534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Theranostics

Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.

Authors - Yumin Li
humanWB,IF

Respir Res

Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1.

Authors - Jin Jin
humanWB

Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani
ratWB,IHC

J Cell Sci

miR-9 inhibits Schwann cell migration by targeting CTHRC1 following sciatic nerve injury.

Authors - Songlin Zhou
  • KD Validated
human,mouseWB,IF

Inflammation

Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro

Authors - Yong Feng
  • KD Validated
WB,IHC

Int J Biol Sci

NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis via inhibiting the CTHRC1/HIF-1α axis.

Authors - Lin Chen