验证数据展示
经过测试的应用
| Positive WB detected in | Con A treated mouse splenocytes |
| Positive IF-P detected in | mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30648-1-AP targets CTLA-4/CD152 in WB, IF-P, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytotoxic T-lymphocyte-associated protein 4 |
| 别名 | CTLA-4, Ctla4, Ly 56, Ctla 4, Cd152 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 28-40 kDa |
| GenBank蛋白编号 | NM_009843.4 |
| 基因名称 | CTLA-4 |
| Gene ID (NCBI) | 12477 |
| RRID | AB_3086380 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09793 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CTLA-4/CD152 antibody 30648-1-AP | Download protocol |
| IF protocol for CTLA-4/CD152 antibody 30648-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |