验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse spleen tissue, mouse thymus tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
13665-1-AP targets CTNNBL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | CTNNBL1 fusion protein Ag4578 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | catenin, beta like 1 |
| 别名 | Beta catenin like protein 1, C20orf33, catenin, beta like 1, CTNNBL1, NAP, Nuclear associated protein, NYD SP19, P14L, PP8304 |
| 计算分子量 | 563 aa, 65 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC036739 |
| 基因名称 | CTNNBL1 |
| Gene ID (NCBI) | 56259 |
| RRID | AB_2086245 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WYA6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTNNBL1, also named as C20orf33, belongs to the armadillo-containing protein family (PMID: 32484799). CTNNBL1 is a widely expressed nucleoprotein that associates with the pre-mRNA processing factor 19 (Prp19) complex of the spliceosome, interacting with its CDC5L component. CTNNBL1 can maintain the content and integrity of the Prp19 spliceosome complex by regulating the interaction between CWC15 and CDC5L (PMID: 28501461). CTNNBL1 has 4 isoforms with the molecular weights of 36, 43, 62 and 65 kDa, respectively. CTNNBL1 may play an important role in colorectal cancer and regulating AID function (PMID: 28501461, 32484799).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CTNNBL1 antibody 13665-1-AP | Download protocol |
| IHC protocol for CTNNBL1 antibody 13665-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Transl Med eIF6 promotes the malignant progression of human hepatocellular carcinoma via the mTOR signaling pathway. |
