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p120 Catenin Polyclonal antibody

p120 Catenin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12180-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

CTNND1, CTNND, Catenin delta-1, Catenin delta 1, CAS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman brain tissue, HeLa cells, HEK-293 cells, mouse brain tissue, NIH/3T3 cells, RAW264.7 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy p120 Catenin Ready-To-Use IHC Kit. p120 Catenin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12180-1-AP targets p120 Catenin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine
免疫原 p120 Catenin fusion protein Ag2824 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), delta 1
别名 CTNND1, CTNND, Catenin delta-1, Catenin delta 1, CAS
计算分子量 948 aa, 105 kDa
观测分子量 90-120 kDa
GenBank蛋白编号BC010501
基因名称 p120 Catenin
Gene ID (NCBI) 1500
RRIDAB_2086267
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60716
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

实验方案

Product Specific Protocols
WB protocol for p120 Catenin antibody 12180-1-APDownload protocol
IHC protocol for p120 Catenin antibody 12180-1-APDownload protocol
IF protocol for p120 Catenin antibody 12180-1-APDownload protocol
IP protocol for p120 Catenin antibody 12180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Hum Behav

Genome-wide meta-analysis, functional genomics and integrative analyses implicate new risk genes and therapeutic targets for anxiety disorders

Authors - Wenqiang Li
mouseWB

EMBO Mol Med

Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy.

Authors - Jie-Hong Wu
mouseWB

EBioMedicine

NDRG2 regulates adherens junction integrity to restrict colitis and tumourigenesis.

Authors - Mengying Wei
mouseWB

CNS Neurosci Ther

The Neuroprotective Mechanisms of PPAR-γ: Inhibition of Microglia-Mediated Neuroinflammation and Oxidative Stress in a Neonatal Mouse Model of Hypoxic-Ischemic White Matter Injury

Authors - Mingchu Fang
humanWB

Cell Cycle

Hsa-miR-425-5p promotes tumor growth and metastasis by activating the CTNND1-mediated β-catenin pathway and EMT in colorectal cancer.

Authors - Dingsheng Liu
human,mouseWB

Front Pharmacol

ALG-bFGF Hydrogel Inhibiting Autophagy Contributes to Protection of Blood-Spinal Cord Barrier Integrity via PI3K/Akt/FOXO1/KLF4 Pathway After SCI.

Authors - Renkan Zhang
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