验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, BxPC-3 cells |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
14856-1-AP targets CTRL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CTRL fusion protein Ag6644 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chymotrypsin-like |
| 别名 | EC:3.4.21.-, CTRL1, Chymotrypsin-like protease CTRL-1, chymotrypsin like |
| 计算分子量 | 28 kDa |
| 观测分子量 | 25-28 kDa |
| GenBank蛋白编号 | BC063475 |
| 基因名称 | CTRL |
| Gene ID (NCBI) | 1506 |
| RRID | AB_10640303 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P40313 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CTRL antibody 14856-1-AP | Download protocol |
| IHC protocol for CTRL antibody 14856-1-AP | Download protocol |
| IF protocol for CTRL antibody 14856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Thromb Haemost Plasma Proteolytic Cascade Activation during Neonatal Cardiopulmonary Bypass Surgery. | ||
Biol Open Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems. | ||
Oncotarget The levels of serine proteases in colon tissue interstitial fluid and serum serve as an indicator of colorectal cancer progression. |
