Cathepsin D Polyclonal antibody
Cathepsin D Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HepG2 cells, MCF-7 cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
IF | See 2 publications below |
产品信息
55021-1-AP targets Cathepsin D in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cathepsin D |
Synonyms | Cathepsin D, CLN10, CPSD, CTSD |
Calculated Molecular Weight | 45 kDa |
Observed Molecular Weight | 32 kDa |
GenBank Accession Number | NM_001909 |
Gene Symbol | Cathepsin D |
Gene ID (NCBI) | 1509 |
RRID | AB_10863128 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P07339 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Cathepsin D antibody 55021-1-AP | Download protocol |
IHC protocol for Cathepsin D antibody 55021-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease | ||
Toxicology 1,3-dichloro-2-propanol induced lipid accumulation by blocking autophagy flux in HepG2 cells. | ||
Pathogens Sputum from People with Cystic Fibrosis Reduces the Killing of Methicillin-Resistant Staphylococcus aureus by Neutrophils and Diminishes Phagosomal Production of Reactive Oxygen Species | ||
Cell Death Discov A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. | ||
Chem Biol Interact In addition to its endosomal escape effect, platycodin D also synergizes with ribosomal inactivation protein to induce apoptosis in hepatoma cells through AKT and MAPK signaling pathways. | ||
Biochem Biophys Res Commun Bifendate inhibits autophagy at multiple steps and attenuates oleic acid-induced lipid accumulation |