验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55021-1-AP targets Cathepsin D in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cathepsin D |
| 别名 | CTSD, Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD |
| 计算分子量 | 45 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | NM_001909 |
| 基因名称 | Cathepsin D |
| Gene ID (NCBI) | 1509 |
| RRID | AB_10863128 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07339 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTSD (Cathepsin D) is also named as CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Cathepsin D antibody 55021-1-AP | Download protocol |
| WB protocol for Cathepsin D antibody 55021-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Repression of RIPK1 kinase by INPP5D inhibits expression of diverse proinflammatory mediators and late-onset Alzheimer's disease risk factors | ||
Phytomedicine Cepharanthine sensitizes gastric cancer cells to chemotherapy by targeting TRIB3-FOXO3-FOXM1 axis to inhibit autophagy | ||
Chin Herb Med Precise subcellular organelle-targeted analyses of hepatotoxicity of Polygonum multiflorum. | ||
Cell Death Discov A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. | ||
Free Radic Biol Med Sestrin2 improves intestinal ischemia-reperfusion injury through the TFEB-mediated autophagy/lysosomal pathway |
