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Cathepsin K Polyclonal antibody

Cathepsin K Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11239-1-AP

Print datasheet

Synonyms

CTSK, EC:3.4.22.38, CTSO2, CTSO, catK



经过测试的应用

Positive WB detected inA549 cells, U-87 MG cells, ROS1728 cells
Positive IP detected inROS1728 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human osteosarcoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11239-1-AP targets Cathepsin K in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Immunogen Cathepsin K fusion protein Ag1761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cathepsin K
Synonyms CTSK, EC:3.4.22.38, CTSO2, CTSO, catK
Calculated Molecular Weight 39 kDa
Observed Molecular Weight27 kDa, 38~46 kDa
GenBank Accession NumberBC016058
Gene Symbol Cathepsin K
Gene ID (NCBI) 1513
RRIDAB_2245581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43235
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTSK(cathepsin K), also named CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts (PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot (PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

实验方案

Product Specific Protocols
WB protocol for Cathepsin K antibody 11239-1-APDownload protocol
IHC protocol for Cathepsin K antibody 11239-1-APDownload protocol
IF protocol for Cathepsin K antibody 11239-1-APDownload protocol
IP protocol for Cathepsin K antibody 11239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Circadian control of the secretory pathway maintains collagen homeostasis.

Authors - Joan Chang
mouseIHC,IF

Biomaterials

BMP2 immune complexes promote new bone formation by facilitating the direct contact between osteoclasts and osteoblasts.

Authors - Yamei Xu
mouseIF

Dev Cell

Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption

Authors - Mobai Li
mouse

ACS Appl Mater Interfaces

Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis

Authors - Wenzheng Liao
mouse

Br J Pharmacol

Andrographolide prevents bone loss via targeting ERRalpha-regulated metabolic adaption of osteoclastogenesis.

Authors - Tongling Huang
mouseIHC

Arthritis Rheum

Osteoarthritic change is delayed in a Ctsk-knockout mouse model of osteoarthritis.

Authors - Kozawa Eiji E