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- KD/KO Validated
Cathepsin K Polyclonal antibody
Cathepsin K Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, U-87 MG cells, ROS1728 cells |
Positive IP detected in | ROS1728 cells |
Positive IHC detected in | human breast cancer tissue, human lung cancer tissue, human osteosarcoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | ROS1728 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11239-1-AP targets Cathepsin K in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish |
Immunogen | Cathepsin K fusion protein Ag1761 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cathepsin K |
Synonyms | CTSK, EC:3.4.22.38, CTSO2, CTSO, catK |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 27 kDa, 38~46 kDa |
GenBank Accession Number | BC016058 |
Gene Symbol | Cathepsin K |
Gene ID (NCBI) | 1513 |
RRID | AB_2245581 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P43235 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Cathepsin K antibody 11239-1-AP | Download protocol |
IHC protocol for Cathepsin K antibody 11239-1-AP | Download protocol |
IF protocol for Cathepsin K antibody 11239-1-AP | Download protocol |
IP protocol for Cathepsin K antibody 11239-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biomaterials BMP2 immune complexes promote new bone formation by facilitating the direct contact between osteoclasts and osteoblasts. | ||
Dev Cell Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption | ||
ACS Appl Mater Interfaces Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis | ||
Br J Pharmacol Andrographolide prevents bone loss via targeting ERRalpha-regulated metabolic adaption of osteoclastogenesis. | ||
Arthritis Rheum Osteoarthritic change is delayed in a Ctsk-knockout mouse model of osteoarthritis. |