验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, human testis tissue, rat liver tissue |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
18442-1-AP targets Cathepsin V in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | Cathepsin V fusion protein Ag13284 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cathepsin L2 |
Synonyms | CTSL2, CTSV, CTSU, CATL2, Cathepsin U |
Calculated Molecular Weight | 37 kDa |
Observed Molecular Weight | 26-36 kDa |
GenBank Accession Number | BC023504 |
Gene Symbol | Cathepsin V |
Gene ID (NCBI) | 1515 |
RRID | AB_10644340 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O60911 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cathepsin V is a lysosomal cysteine protease that has an important role in corneal physiology. Cathepsin V was found to be differentially expressed in relation to pigmentary skin backgrounds, and was about 7.5-folds higher in keratinocytes from lightly pigmented, relative to darkly pigmented skins. It is expressed in primary keratinocytes, melanocytes, and HaCaT keratinocytes, but not in primary fibroblasts. The purified cathepsin L2 was a high molecular weight proteinase of about 78 kDa, but it was unstable to SDS and reducing conditions, which dissociated parts of the proteinase into 66 kDa, 31 kDa and 26 kDa fragments(Food Chemistry,Volume11,Issue4,879-886) and it can form a complex of 70 kDa with an unidentified subunit(PMID:1930136).
实验方案
Product Specific Protocols | |
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WB protocol for Cathepsin V antibody 18442-1-AP | Download protocol |
IHC protocol for Cathepsin V antibody 18442-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |