Cathepsin V Polyclonal antibody

Cathepsin V Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18442-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

CTSL2, CTSV, CTSU, CATL2, Cathepsin U

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, rat liver tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

18442-1-AP targets Cathepsin V in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Cathepsin V fusion protein Ag13284 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathepsin L2
别名 CTSL2, CTSV, CTSU, CATL2, Cathepsin U
计算分子量 37 kDa
观测分子量26-36 kDa
GenBank蛋白编号BC023504
基因名称 Cathepsin V
Gene ID (NCBI) 1515
RRIDAB_10644340
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60911
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cathepsin V is a lysosomal cysteine protease that has an important role in corneal physiology. Cathepsin V was found to be differentially expressed in relation to pigmentary skin backgrounds, and was about 7.5-folds higher in keratinocytes from lightly pigmented, relative to darkly pigmented skins. It is expressed in primary keratinocytes, melanocytes, and HaCaT keratinocytes, but not in primary fibroblasts. The purified cathepsin L2 was a high molecular weight proteinase of about 78 kDa, but it was unstable to SDS and reducing conditions, which dissociated parts of the proteinase into 66 kDa, 31 kDa and 26 kDa fragments(Food Chemistry,Volume11,Issue4,879-886) and it can form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

实验方案

Product Specific Protocols
WB protocol for Cathepsin V antibody 18442-1-APDownload protocol
IHC protocol for Cathepsin V antibody 18442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Mol Carcinog

Cathepsin V is correlated with the prognosis and tumor microenvironment in liver cancer

Authors - Junyu Liu
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