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CTSO Polyclonal antibody

CTSO Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14296-1-AP

Print datasheet

Synonyms

cathepsin O, CTSO, CTSO1

验证数据展示

  • IHC staining of human ovary tumor using 14296-1-AP

    IHC staining of human ovary tumor using 14296-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14296-1-AP (CTSO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 14296-1-AP

    IHC staining of human ovary tumor using 14296-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14296-1-AP (CTSO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293 using 14296-1-AP

    IP experiment of HEK-293 using 14296-1-AP

    IP result of anti-CTSO (IP:14296-1-AP, 4ug; Detection:14296-1-AP 1:300) with HEK-293 cells lysate 3600ug.

经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14296-1-AP targets CTSO in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CTSO fusion protein Ag5651 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cathepsin O
Synonyms cathepsin O, CTSO, CTSO1
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC049206
Gene Symbol CTSO
Gene ID (NCBI) 1519
RRIDAB_2878042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43234
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CTSO antibody 14296-1-APDownload protocol
IP protocol for CTSO antibody 14296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols