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CTSS Polyclonal antibody

CTSS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27538-1-AP

Print datasheet

Synonyms

EC:3.4.22.27, Cathepsin S

验证数据展示

  • WB analysis using 27538-1-AP

    WB analysis using 27538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27538-1-AP (CTSS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27538-1-AP

    IHC staining of human colon cancer using 27538-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27538-1-AP (CTSS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27538-1-AP

    IHC staining of human colon cancer using 27538-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27538-1-AP (CTSS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-87 MG using 27538-1-AP

    IF Staining of U-87 MG using 27538-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using CTSS antibody (27538-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHUVEC cells, THP-1 cells, U-87 MG cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27538-1-AP targets CTSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CTSS fusion protein Ag25881 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cathepsin S
Synonyms EC:3.4.22.27, Cathepsin S
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 25 kDa, 37 kDa
GenBank Accession NumberBC002642
Gene Symbol CTSS
Gene ID (NCBI) 1520
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25774
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cathepsin S (CTSS) is a cysteine protease that plays an important role in extracellular matrix degradation and remodeling, antigen presentation, inflammation, and angiogenesis; it has been identified as a radiation response gene. In malignant tumors, CTSS induces tumor proliferation, invasion and metastasis through various mechanisms. CTSS proteins could be detected in two forms, pro-CTSS (35-37 kDa) and active CTSS (25 kDa) (PMID: 30006610, PMID: 34208434). And in this publication, it was also reporeted that active CTSS was expressed at higher levels than pro-CTSS in the lysate (PMID: 34208434).

实验方案

Product Specific Protocols
WB protocol for CTSS antibody 27538-1-APDownload protocol
IHC protocol for CTSS antibody 27538-1-APDownload protocol
IF protocol for CTSS antibody 27538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols