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验证数据展示

  • WB analysis using 20123-1-AP

    WB analysis using 20123-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 20123-1-AP

    IP experiment of mouse brain using 20123-1-AP

    IP result of anti-CUEDC2 (IP:20123-1-AP, 4ug; Detection:20123-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • IHC staining of human ovary tumor using 20123-1-AP

    IHC staining of human ovary tumor using 20123-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 20123-1-AP

    IHC staining of human ovary tumor using 20123-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 20123-1-AP

    IF Staining of HeLa using 20123-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUEDC2 antibody (20123-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CUEDC2 Polyclonal antibody

CUEDC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20123-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

HOYS6, CUE domain-containing protein 2, CUE domain containing 2, C10orf66

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse kidney tissue, HeLa cells, human brain tissue, human kidney tissue, Jurkat cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20123-1-AP targets CUEDC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 CUEDC2 fusion protein Ag13847 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CUE domain containing 2
别名 HOYS6, CUE domain-containing protein 2, CUE domain containing 2, C10orf66
计算分子量 287 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC000262
基因名称 CUEDC2
Gene ID (NCBI) 79004
RRIDAB_10638318
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H467
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif-containing protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis. It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers, such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) for dephosphorylation and inactivation by recruiting protein phosphatase (PP1), and thus repressed activation of the transcription factor NF-κB. Moreover, CUEDC2, is a key factor in endocrine resistance in breast cancer.

实验方案

Product Specific Protocols
WB protocol for CUEDC2 antibody 20123-1-APDownload protocol
IHC protocol for CUEDC2 antibody 20123-1-APDownload protocol
IF protocol for CUEDC2 antibody 20123-1-APDownload protocol
IP protocol for CUEDC2 antibody 20123-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols