验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse thymus tissue, K-562 cells, MCF-7 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12921-1-AP targets CUGBP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | CUGBP2 fusion protein Ag3594 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CUG triplet repeat, RNA binding protein 2 |
| 别名 | CELF-2, CELF2, CELF 2, Bruno-like protein 3, BRUNOL3 |
| 计算分子量 | 56 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC036391 |
| 基因名称 | CUGBP2 |
| Gene ID (NCBI) | 10659 |
| RRID | AB_2230041 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95319 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The CELF (CUGBP and ETR-3 Like Factor)/Bruno-like protein family plays important roles in the regulation of alternative splicing and translation. CUGBP2 is a member of this family of proteins that possesses several alternatively spliced exons. CUGBP2 induces smooth muscle-specific exon inclusion via binding to uridine purine repeat elements (URE) in ACTN1. Although CUGBP2 is known as a splicing activator for the N-methyl-D-aspartate receptor 1 (NMDA R1) exon 21, CUGBP2 represses NMDA R1 exon 5 inclusion. In addition, CUGBP2 was reported to repress the inclusion of the Ir exon 11.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CUGBP2 antibody 12921-1-AP | Download protocol |
| IHC protocol for CUGBP2 antibody 12921-1-AP | Download protocol |
| IF protocol for CUGBP2 antibody 12921-1-AP | Download protocol |
| IP protocol for CUGBP2 antibody 12921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells. | ||
Brain Res Transcriptional induction and translational inhibition of Arc and Cugbp2 in mice hippocampus after transient global ischemia under normothermic condition. | ||
Exp Biol Med (Maywood) Hypoxic colorectal cancer-secreted exosomes deliver miR-210-3p to normoxic tumor cells to elicit a protumoral effect. | ||
Pharmacol Res Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway | ||
bioRxiv LINC01432 binds to CELF2 in newly diagnosed multiple myeloma promoting short progression-free survival to standard therapy |
