验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, Raji cells, NIH/3T3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30071-1-AP targets CUL2 in WB, ELISA applications and shows reactivity with Human, mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, mouse |
免疫原 | CUL2 fusion protein Ag32331 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cullin 2 |
别名 | CUL 2, CUL2, cullin 2 |
计算分子量 | 87 kDa |
观测分子量 | 80-87 kDa |
GenBank蛋白编号 | BC009591 |
基因名称 | CUL2 |
Gene ID (NCBI) | 8453 |
RRID | AB_3086224 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13617 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
实验方案
Product Specific Protocols | |
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WB protocol for CUL2 antibody 30071-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |