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CUL3 Polyclonal antibody

CUL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28087-1-AP

Print datasheet

Synonyms

CUL 3, CUL3, cullin 3, KIAA0617

验证数据展示

  • WB analysis using 28087-1-AP

    WB analysis using 28087-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28087-1-AP (CUL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 28087-1-AP

    IHC staining of human prostate cancer using 28087-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28087-1-AP (CUL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CUL3 antibody included

经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CUL3 Ready-To-Use IHC Kit. CUL3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28087-1-AP targets CUL3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CUL3 fusion protein Ag28004 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cullin 3
Synonyms CUL 3, CUL3, cullin 3, KIAA0617
Calculated Molecular Weight 89 kDa
Observed Molecular Weight80-88 kDa
GenBank Accession NumberBC039598
Gene Symbol CUL3
Gene ID (NCBI) 8452
RRIDAB_2918147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13618
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.

实验方案

Product Specific Protocols
WB protocol for CUL3 antibody 28087-1-APDownload protocol
IHC protocol for CUL3 antibody 28087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols