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CUTC Polyclonal antibody

CUTC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15030-1-AP

Print datasheet

Synonyms

CGI-32

验证数据展示

  • WB analysis of HeLa using 15030-1-AP

    WB analysis of HeLa using 15030-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15030-1-AP (CUTC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 15030-1-AP

    IHC staining of human endometrial cancer using 15030-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15030-1-AP (CUTC antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human endometrial cancer using 15030-1-AP

    IHC staining of human endometrial cancer using 15030-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15030-1-AP (CUTC antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 15030-1-AP

    IF Staining of HeLa using 15030-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUTC antibody (15030-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, mouse skeletal muscle tissue
Positive IHC detected inhuman endometrial cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15030-1-AP targets CUTC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CUTC fusion protein Ag2126 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cutC copper transporter homolog (E. coli)
Synonyms CGI-32
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC021105
Gene Symbol CUTC
Gene ID (NCBI) 51076
RRIDAB_2086984
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NTM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CUTC antibody 15030-1-APDownload protocol
IHC protocol for CUTC antibody 15030-1-APDownload protocol
IF protocol for CUTC antibody 15030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols