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CWC22 Polyclonal antibody

CWC22 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26898-1-AP

Print datasheet

Synonyms

Pre-mRNA-splicing factor CWC22 homolog, NCM, fSAPb, EIF4GL

验证数据展示

  • WB analysis of A549 using 26898-1-AP

    WB analysis of A549 using 26898-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26898-1-AP (CWC22 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 26898-1-AP

    IHC staining of human stomach using 26898-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26898-1-AP (CWC22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 26898-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26898-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26898-1-AP (CWC22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26898-1-AP targets CWC22 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CWC22 fusion protein Ag25466 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CWC22 spliceosome-associated protein homolog (S. cerevisiae)
Synonyms Pre-mRNA-splicing factor CWC22 homolog, NCM, fSAPb, EIF4GL
Calculated Molecular Weight 908 aa, 106 kDa
Observed Molecular Weight 105-110 kDa
GenBank Accession NumberBC093954
Gene Symbol CWC22
Gene ID (NCBI) 57703
RRIDAB_2880674
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCG8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CWC22, Pre-mRNA-splicing factor CWC22 homolog, is a 908 amino acid protein, which contains 1 MI domain and 1 MIF4G domain and belongs to the CWC22 family. CWC22 is required for pre-mRNA splicing and for exon-junction complex (EJC) assembly and hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs.

实验方案

Product Specific Protocols
WB protocol for CWC22 antibody 26898-1-APDownload protocol
IHC protocol for CWC22 antibody 26898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation.

Authors - Cole Townsend
View Article