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验证数据展示

  • WB analysis of A549 using 26898-1-AP

    WB analysis of A549 using 26898-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26898-1-AP (CWC22 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 26898-1-AP

    IHC staining of human stomach using 26898-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26898-1-AP (CWC22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 26898-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26898-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26898-1-AP (CWC22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CWC22 Polyclonal antibody

CWC22 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26898-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Pre-mRNA-splicing factor CWC22 homolog, NCM, fSAPb, EIF4GL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26898-1-AP targets CWC22 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 CWC22 fusion protein Ag25466 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CWC22 spliceosome-associated protein homolog (S. cerevisiae)
别名 Pre-mRNA-splicing factor CWC22 homolog, NCM, fSAPb, EIF4GL
计算分子量 908 aa, 106 kDa
观测分子量 105-110 kDa
GenBank蛋白编号BC093954
基因名称 CWC22
Gene ID (NCBI) 57703
RRIDAB_2880674
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCG8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CWC22, Pre-mRNA-splicing factor CWC22 homolog, is a 908 amino acid protein, which contains 1 MI domain and 1 MIF4G domain and belongs to the CWC22 family. CWC22 is required for pre-mRNA splicing and for exon-junction complex (EJC) assembly and hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs.

实验方案

Product Specific Protocols
WB protocol for CWC22 antibody 26898-1-APDownload protocol
IHC protocol for CWC22 antibody 26898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation.

Authors - Cole Townsend
View Article