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CX3CR1 Polyclonal antibody

CX3CR1 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29819-1-AP

Print datasheet

Synonyms

Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, RBS11, V28

验证数据展示

  • IF Staining of mouse kidney using 29819-1-AP

    IF Staining of mouse kidney using 29819-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CX3CR1 antibody (29819-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 29819-1-AP

    WB analysis using 29819-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29819-1-AP (CX3CR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, THP-1 cells, Caco-2 cells
Positive IF-P detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29819-1-AP targets CX3CR1 in WB, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity Human
Immunogen CX3CR1 fusion protein Ag27698 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-X3-C motif) receptor 1
Synonyms Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, RBS11, V28
Calculated Molecular Weight 40 kDa
Observed Molecular Weight40 kDa, 44 kDa
GenBank Accession NumberBC028078
Gene Symbol CX3CR1
Gene ID (NCBI) 1524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49238
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12). The two CX3CR1 bands exhibited can be attributed to various factors (PMID: 30628679).

实验方案

Product Specific Protocols
WB protocol for CX3CR1 antibody 29819-1-APDownload protocol
IF protocol for CX3CR1 antibody 29819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols