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CX3CR1 Monoclonal antibody, PBS Only

CX3CR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3D3B8

Cat no : 66915-1-PBS

Print datasheet

Synonyms

Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, RBS11, V28

验证数据展示

  • IHC staining of mouse kidney using 66915-1-Ig (same clone as 66915-1-PBS)

    IHC staining of mouse kidney using 66915-1-Ig (same clone as 66915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66915-1-Ig (CX3CR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 66915-1-Ig (same clone as 66915-1-PBS)

    IF Staining of mouse kidney using 66915-1-Ig (same clone as 66915-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CX3CR1 antibody (66915-1-Ig, Clone: 3D3B8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

  • WB analysis using 66915-1-Ig (same clone as 66915-1-PBS)

    WB analysis using 66915-1-Ig (same clone as 66915-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66915-1-Ig (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66915-1-PBS in a different storage buffer formulation.

产品信息

66915-1-PBS targets CX3CR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human
Immunogen CX3CR1 fusion protein Ag27698 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chemokine (C-X3-C motif) receptor 1
Synonyms Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, RBS11, V28
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC028078
Gene Symbol CX3CR1
Gene ID (NCBI) 1524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49238
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).