• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 98265-1-RR

    FC experiment of human PBMCs using 98265-1-RR

    1x10^6 untreated or IFN gamma and brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL10/IP-10 Rabbit Recombinant Antibody (98265-1-RR, Clone: 242013E12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Anti-Human CXCL10/IP-10 Rabbit Recombinant Antibody

CXCL10/IP-10 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98265-1-RR

Print datasheet

CloneNo. 242013E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CXCL10, CXCL10/IP10, IP10, CXCL 10, C-X-C motif chemokine 10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inIFN gamma and brefeldin A treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy CXCL10/IP10 Ready-To-Use IHC Kit. CXCL10/IP10 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98265-1-RR targets CXCL10/IP-10 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-X-C motif) ligand 10
Synonyms CXCL10, CXCL10/IP10, IP10, CXCL 10, C-X-C motif chemokine 10
Calculated Molecular Weight 11 kDa
GenBank Accession NumberBC010954
Gene Symbol CXCL10
Gene ID (NCBI) 3627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02778
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CXCL10 (also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.

实验方案

Product Specific Protocols
FC protocol for CXCL10/IP-10 antibody 98265-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols