CXCL8/IL-8 Recombinant antibody

CXCL8/IL-8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83903-4-RR

产品说明书

CloneNo. 240889C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells
Positive IF/ICC detected inPMA and Brefeldin A treated THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy CXCL8/IL-8 Ready-To-Use IHC Kit. CXCL8/IL-8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83903-4-RR targets CXCL8/IL-8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 8
别名 CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif
计算分子量 99 aa, 11 kDa
观测分子量11 kDa
GenBank蛋白编号BC013615
基因名称 IL-8
Gene ID (NCBI) 3576
ENSEMBL Gene IDENSG00000169429
RRIDAB_3671485
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10145
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa).

实验方案

Product Specific Protocols
WB protocol for CXCL8/IL-8 antibody 83903-4-RRDownload protocol
IF protocol for CXCL8/IL-8 antibody 83903-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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