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CXCL8/IL-8 Recombinant antibody, PBS Only (Detector)

CXCL8/IL-8 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240889C2

Cat no : 83903-1-PBS

Print datasheet

Synonyms

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

验证数据展示

  • Sandwich ELISA standard curve of MP00830-3

    Sandwich ELISA standard curve of MP00830-3

    Sandwich ELISA standard curve of MP00830-3, Human CXCL8/IL-8 Recombinant Matched Antibody Pair - PBS only. 83903-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0152. 83903-1-PBS was HRP conjugated as the detection antibody. Range: 2.73-175 pg/mL.

  • Sample test of MP00830-3

    Sample test of MP00830-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 or 5 days. For the PBMC supernatant cultured for 3 days, the mean CXCL8/IL-8 concentration was determined to be 4.46 ng/mL in unstimulated PBMC supernatant, 252.88 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean CXCL8/IL-8 concentration was determined to be 317.16 ng/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00830-3

    Sample test of MP00830-3

    Serum of sixteen individual healthy human donors was measured. The CXCL8/IL-8 concentration of detected samples was determined to be 29.8 pg/mL with a range of 8.9 - 104.1 pg/mL.

  • Cytometric bead array standard curve of MP00830-1

    Cytometric bead array standard curve of MP00830-1

    Cytometric bead array standard curve of MP00830-1, CXCL8/IL-8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83903-3-PBS. Detection antibody: 83903-1-PBS. Standard: Eg0152. Range: 0.625-40 ng/mL.

  • Cytometric bead array standard curve of MP00830-2

    Cytometric bead array standard curve of MP00830-2

    Cytometric bead array standard curve of MP00830-2, CXCL8/IL-8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83903-2-PBS. Detection antibody: 83903-1-PBS. Standard: Eg0152. Range: 0.625-40 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CXCL8/IL-8 Ready-To-Use IHC Kit. CXCL8/IL-8 primary antibody included.

产品信息

83903-1-PBS targets CXCL8/IL-8 as part of a matched antibody pair:

MP00830-1: 83903-3-PBS capture and 83903-1-PBS detection (validated in Cytometric bead array)

MP00830-2: 83903-2-PBS capture and 83903-1-PBS detection (validated in Cytometric bead array)

MP00830-3: 83903-5-PBS capture and 83903-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 8
Synonyms CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif
Calculated Molecular Weight 99 aa, 11 kDa
GenBank Accession NumberBC013615
Gene Symbol IL-8
Gene ID (NCBI) 3576
ENSEMBL Gene IDENSG00000169429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10145
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C