验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55450-1-AP targets CXCR1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 8 receptor, alpha |
| 别名 | IL8RA, CKR 1, CDw128a, CD181, CD128 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | NM_000634 |
| 基因名称 | CXCR1 |
| Gene ID (NCBI) | 3577 |
| RRID | AB_11182706 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P25024 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CXCR1, also named CMKAR1, IL8RA, and CD181, belongs to the G-protein coupled receptor 1 family. It is a receptor to interleukin-8, a powerful neutrophil chemotactic factor. The binding of IL-8 to the receptor causes activation of neutrophils. CXCR1 binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. This antibody is specific to CXCR1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CXCR1 antibody 55450-1-AP | Download protocol |
| IF protocol for CXCR1 antibody 55450-1-AP | Download protocol |
| IP protocol for CXCR1 antibody 55450-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis CXCR1 drives the pathogenesis of EAE and ARDS via boosting dendritic cells-dependent inflammation | ||
Cell Rep Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism
| ||
Lung Cancer Combination therapy with anti-programmed cell death 1 antibody plus angiokinase inhibitor exerts synergistic antitumor effect against malignant mesothelioma via tumor microenvironment modulation | ||
Cytokine IL-8 exacerbates alcohol-induced fatty liver disease via the Akt/HIF-1α pathway in human IL-8-expressing mice. | ||
J Agric Food Chem Oral Intervention of Narirutin Ameliorates the Allergic Response of Ovalbumin Allergy | ||
Mol Immunol IL-8 exacerbates CCl4-induced liver fibrosis in human IL-8-expressing mice via the PI3K/Akt/HIF-1α pathway |